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The influence of nanopillar structure size to fibroblast’s proliferation and alpha-smooth muscle actin (α-SMA) expression
J Korean Skull Base Soc 2020;15(2):98-104
Published online October 31, 2020
© 2020 Korean Skull Base Society.

Eun Hye Kang1, Sang Soo Lee1,2, Young Sik Yun3,4, Won Jai Lee1, Jong Souk Yeo3,4, Tai Suk Roh1,2, In Sik Yun1,2

1Department of Plastic and Reconstructive Surgery, Yonsei University College of Medicine, Seoul, Korea
2Department of Plastic and Reconstructive Surgery, Gangnam Severance Hospital, Seoul, Korea
3School of Integrated Technology, Yonsei University, Incheon, Korea
4Yonsei Institute of Convergence Technology, Yonsei University, Incheon, Korea
Correspondence to: In Sik Yun
주소 : Department of Plastic and Reconstructive Surgery, Gangnam Severance Hospital, Yonsei University College of Medicine, 211 Eonju-ro, Gangnam-gu, Seoul, Korea
Tel : +82-2-2019-3423
Fax : +82-2-2019-4881
E-mail : eqatom@yuhs.ac
Received August 28, 2020; Accepted October 2, 2020.
Abstract
Background : With advances in medicine and materials engineering, biomaterials, such as silicone and titanium are used in many tissue regeneration cases. Many studies are being conducted to confirm the reactivity of cells to various biomaterials. Among these studies, fibroblast proliferation and differentiation are known as important factors related to capsulation. In this study, we attempted to determine how various sized polyurethane acrylate (PUA) nanopillar structures act on fibroblast proliferation and differentiation.
Methods : The scaffolds were prepared in four groups: flat and nanopillar (350 nm, 500 nm, 1,000 nm). Flat group was used as a control. To evaluate the effect of scaffolds of nanopillar structures on fibroblasts, quantitatively analyzed for the degree of adhesion, proliferation and alpha-smooth muscle actin (α-SMA) expression of cells.
Results : As a result of the MTT assay, the proliferation was lower than that of the flat group in the nanopillar group. As a result of staining, the intensity of F-actin was significantly different from that of the flat group. And the intensity of α-SMA was different from that of the flat group but not significantly.
Conclusions : The nanopillar structure of PUA material seems to play a role in inhibiting the proliferation and differentiation of fibroblasts regardless of the size of the structure. This suggests that the use of PUA nanopillar implants may be effective in suppressing fibrosis due to fibroblast proliferation and differentiation.
Keywords : Nanopillar, Fibroblast, Cell attachment, Cell proliferation


October 2020, 15 (2)
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